AUTHOR=Khamwut Ariya , Nimnual Juthamas , Chomta Nantinee , Nimsamer Pattaraporn , Mayuramart Oraphan , Kaewsapsak Pornchai , Pasittungkul Siripat , Poovorawan Yong , Payungporn Sunchai 

TITLE=Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a

JOURNAL=Experimental Biology and Medicine

VOLUME=Volume 250 - 2025

YEAR=2025

URL=https://www.ebm-journal.org/journals/experimental-biology-and-medicine/articles/10.3389/ebm.2025.10387

DOI=10.3389/ebm.2025.10387

ISSN=1535-3699

ABSTRACT=Human respiratory syncytial virus (hRSV) is one of the most prevalent viruses infecting children globally. In this study, we employed the RT-RPA with CRISPR/Cas12a detection methodology to detect and differentiate RSV-A and RSV-B, particularly in resource-limited settings. The detection limit for RSV-A and RSV-B was approximately 102 and 103 copies/reaction, respectively. The assay revealed 100% specificity in detecting both RSV-A and RSV-B. Diagnostic accuracy was 90.32 and 93.55% for RSV-A and RSV-B, respectively, compared to RT-qPCR. These data indicate a proficient strategy for RSV screening, demonstrating promise for prospective applications in detecting diverse viral infections.