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        <title>Experimental Biology and Medicine | Stem Cell Biology section | New and Recent Articles</title>
        <link>https://www.ebm-journal.org/journals/experimental-biology-and-medicine/sections/stem-cell-biology</link>
        <description>RSS Feed for Stem Cell Biology section in the Experimental Biology and Medicine journal | New and Recent Articles</description>
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        <pubDate>2026-04-08T13:01:11.529+00:00</pubDate>
        <ttl>60</ttl>
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        <guid isPermaLink="true">https://www.ebm-journal.org/articles/10.3389/ebm.2025.10611</guid>
        <link>https://www.ebm-journal.org/articles/10.3389/ebm.2025.10611</link>
        <title><![CDATA[Csf1+ AD-MSCs promote stroke repair by activating the resident microglia]]></title>
        <pubdate>2025-08-20T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Jiguang Hou</author><author>Sunfu Zhang</author><author>Shuang Luo</author><author>Xiao Zuo</author><author>Fei Ma</author><author>Huizhen Wang</author><author>Pengfei Han</author><author>Ping Zhu</author><author>Ning Wang</author><author>Xiaoming Hou</author><author>Jin Li</author>
        <description><![CDATA[The potential of mesenchymal stromal cells (MSCs) in the treatment of hemorrhagic stroke has been demonstrated; however, their clinical efficacy remains inconsistent and further comprehensive studies on their mechanism of action are warranted. In this study, the intracerebral hemorrhage (ICH) rat model was used for intravenous infusion of adipose-derived mesenchymal stromal cells (AD-MSCs) 24 h after modeling. Histopathological techniques and single cell transcriptome sequencing techniques were used to study the mechanism of AD-MSCs promoting the repair of damaged brain tissue. The results indicated that AD-MSCs markedly promote the repair of damaged brain tissues and restored neural function. Single-cell transcriptome sequencing further revealed that this therapeutic effect is specifically through the inhibition of monocyte infiltration in injured brain tissue, promotion of resident microglia proliferation and signaling pathways linked to immune response and neuroprotection. These processes are closely tied to the Csf1+ subgroup of AD-MSCs. For acute hemorrhagic stroke, Csf1+ AD-MSCs promote the repair of damaged brain tissue by activating resident microglia and inhibiting monocyte infiltration. This study offers novel insights into the mechanisms underlying MSC-based stroke treatment and supports the potential for stable and efficacious MSC therapies.]]></description>
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        <guid isPermaLink="true">https://www.ebm-journal.org/articles/10.3389/ebm.2025.10342</guid>
        <link>https://www.ebm-journal.org/articles/10.3389/ebm.2025.10342</link>
        <title><![CDATA[Induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapy]]></title>
        <pubdate>2025-02-03T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Hemanathan Vembuli</author><author>Sheeja Rajasingh</author><author>Patrick Nabholz</author><author>Jefferson Guenther</author><author>Brian R. Morrow</author><author>Margaret M. Taylor</author><author>Marziyeh Aghazadeh</author><author>Vinoth Sigamani</author><author>Johnson Rajasingh</author>
        <description><![CDATA[Bone fractures and bone loss represent significant global health challenges, with their incidence rising due to an aging population. Despite autologous bone grafts remain the gold standard for treatment, challenges such as limited bone availability, immune reactions, and the risk of infectious disease transmission have driven the search for alternative cell-based therapies for bone regeneration. Stem cells derived from oral tissues and umbilical cord mesenchymal stem cells (MSCs) have shown potential in both preclinical and clinical studies for bone tissue regeneration. However, their limited differentiation capacity and wound healing abilities necessitate the exploration of alternative cell sources. In this study, we generated induced pluripotent stem cells (iPSCs) using a safe, nonviral and mRNA-based approach from human periodontal ligament fibroblasts (PDLF), an easily accessible cell source. These iPSCs were subsequently differentiated into MSCs, referred to as induced MSCs (iMSCs). The resulting iMSCs were homogeneous, highly proliferative, and possessed anti-inflammatory properties, suggesting their potential as a superior alternative to traditional MSCs for regenerative therapy. These iMSCs demonstrated trilineage differentiation potential, giving rise to osteocytes, chondrocytes, and adipocytes. The iMSC-derived osteocytes (iOSTs) were homogeneous, patient-specific and showed excellent attachment and growth on commercial collagen-based membranes, highlighting their suitability for bone tissue regeneration applications. Given their promising characteristics compared to traditional MSCs, PDLF-derived iMSCs are strong candidates for future clinical studies in bone regeneration and other regenerative dental therapies.]]></description>
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